Coding

Part:BBa_J72026:Design

Designed by: Jennifer Brophy   Group: Anderson Lab   (2010-07-15)


{Bmll36} in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 69


Design Notes

N/A


Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll36 {rbs_at_10000.V5>}

Wobble mll36/mll37                (109, EcoRI/BamHI
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll36         {rbs_at_10000.V5>}
----
mll36    Forward wobble for {rbs_at_10000.V5>} (Bmll36)
CCATAGAATTCATGAGATCTGAAGCAATATCTTCACGTATAAGGAGGTATTTCATGGGTAAGCCTATC
mll37    Reverse wobble for {rbs_at_10000.V5>} (Bmll36)
CGTTAGGATCCCGTAGAATCGAGACCCAGCAGAGGGTTAGGGATAGGCTTACCCATGAAATAC


References