Coding
Part:BBa_J72026:Design
Designed by: Jennifer Brophy Group: Anderson Lab (2010-07-15)
{Bmll36} in BBb
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 69
Design Notes
N/A
Source
Overlap Extension PCR (Wobble Construction)
Wobble construction of Bmll36 {rbs_at_10000.V5>}
Wobble mll36/mll37 (109, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-Bmll36 {rbs_at_10000.V5>} ---- mll36 Forward wobble for {rbs_at_10000.V5>} (Bmll36) CCATAGAATTCATGAGATCTGAAGCAATATCTTCACGTATAAGGAGGTATTTCATGGGTAAGCCTATC mll37 Reverse wobble for {rbs_at_10000.V5>} (Bmll36) CGTTAGGATCCCGTAGAATCGAGACCCAGCAGAGGGTTAGGGATAGGCTTACCCATGAAATAC